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Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii


Citation

Bhassu, Subha and See, Leng Min and Hassan, Rosly and Siraj, Siti Shapor and Tan, Soon Guan (2008) Isolation and characterization of microsatellite loci in the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. Molecular Ecology Resources, 8 (5). pp. 983-985. ISSN 1755-098X; ESSN: 1755-0998

Abstract

Eight single locus microsatellite markers were developed to characterize the Malaysian giant freshwater prawn, Macrobrachium rosenbergii. These microsatellites were isolated from an enriched genomic library contained by using a 5′‐anchored polymerase chain reaction technique. Primers were designed to flank the repeat sequences and subsequently used to characterize 30 unrelated individuals of the giant freshwater prawn. The polymerase chain reaction amplification products of these eight microsatellite loci were polymorphic with the number of alleles ranging from two to 10 alleles per locus while the levels of heterozygosity ranged from 0.6333 to 0.8667.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1111/j.1755-0998.2008.02127.x
Publisher: Blackwell Publishing
Keywords: 5'-anchored polymerase chain reaction; Heterozygosity; Macrobrachium rosenbergii; Microsatellite loci; Polymorphisms; RAMs
Depositing User: Nabilah Mustapa
Date Deposited: 08 Jul 2019 07:28
Last Modified: 08 Jul 2019 07:28
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1111/j.1755-0998.2008.02127.x
URI: http://psasir.upm.edu.my/id/eprint/15327
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