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Determination of experimental domain factors of polyphenols, phenolic acids and flavonoids of lemon (Citrus limon) peel using two-level factorial design


Citation

Zainol, Haida and Ab Ghani, Sharin and Nakasha, Jaafar Juju and Hakiman, Mansor (2021) Determination of experimental domain factors of polyphenols, phenolic acids and flavonoids of lemon (Citrus limon) peel using two-level factorial design. Saudi Journal of Biological Sciences, 29 (1). pp. 574-582. ISSN 1319-562X

Abstract

This study aimed to evaluate the significant extraction factors in achieving higher recovery yield of total polyphenols, phenolic acids and flavonoids content from Citrus limon peel using two-level factorial design. The effect of five independent factors including drying temperature (40–60 °C), methanol concentration (20–60%), extraction temperature (28–60 °C), extraction time (30–60 min) and storage duration (0–14 days) were evaluated. Among all the examined factors, results showed that drying temperature, storage duration and extraction temperature were the most significant and contributing factors affecting the total polyphenols, phenolic acids and flavonoids content of lemon peel at P < 0.05. On the contrary, methanol concentration and extraction time exhibited the least significant and contribution at P greater than 0.05. In conclusion, the experimental domain factors were successfully obtained from this experiment, Therefore, further study on optimization of the obtained factors will be conducted in the future study using response surface methodology.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.1016/j.sjbs.2021.09.022
Publisher: Elsevier
Keywords: Citrus limon; Polyphenols; Phenolic acids; Flavonoids; Two-level factorial design
Depositing User: Ms. Nuraida Ibrahim
Date Deposited: 14 Dec 2022 13:26
Last Modified: 14 Dec 2022 13:26
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.sjbs.2021.09.022
URI: http://psasir.upm.edu.my/id/eprint/96656
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