Citation
Soo, Ee Lin
(2003)
Enzymatic Synthesis of Palm Based Amino Acid Esters.
Doctoral thesis, Universiti Putra Malaysia.
Abstract
Acyllysines were successfully prepared from reactions of free fatty acids (FFAs)
(palmitic acid, PA; oleic acid, OA), triglycerides (TGs) (tripalmitin, TP ; triolein, TO)
and palm oil fractions (palm olein, PO; palm kernel olein, PK O) with the unprotected
amino acid, L-lysine, using enzymes as biocatalyst in an organic solvent system. Such a
selective enzymatic route obviates the fastidious regime of substrate
activation/protection required in chemical synthesis. Preliminary detection and
identification of reaction products were facilitated by thin layer chromatography (TLC)
while subsequent quantitative studies on main and interactive effects of parameters
governing the reactions were conducted with TLC-photodensitometry.
Enzyme screening revealed Lipozyme IM to be the most efficient biocatalyst for the
reactions. By varying one-parameter-at-a-time, 70°C was shown to be the optimal
temperature, hexane, the most suitable solvent, and, 5:1 (PA:L-lysine), 3:1 (OA:L-lysine), 1:1 (TP:L-lysine), 1:3 (TO:L-lysine), 1:1 (pO:L-lysine) and 1:I(PKO:L-lysine)
the respective optimal substrate molar ratios. To reach equilibrium, reactions of FF As
and TGs with L-lysine required 4 days while those of the more complex PO and PKO
required 6 days. Higher biocatalyst amounts improved all reactions (except that of TP
with L-lysine), as did higher amounts of molecular sieves added to reactions ofOA and
TO with L-lysine.
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