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Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana)


Citation

Md Saleh, Nur Izzati and Wan Ab Karim Ghani, Wan Azlina and Mustapa Kamal, Siti Mazlina and Harun, Razif (2021) Performance of single and two-stage cross-flow ultrafiltration membrane in fractionation of peptide from microalgae protein hydrolysate (nannochloropsis gaditana). Processes, 9 (4). art. no. 610. pp. 1-20. ISSN 2227-9717

Abstract

Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) and two-stage (10/5 kDa) in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect of flow rate, trans-membrane pressure (TMP), and pH in fractionating MPH were evaluated based on permeate flux and peptide transmission. The results showed that, for single UF membrane, optimum operating parameters were at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 9, with permeate flux of 43.65 L/m2 h (10 kDa) and 55.42 L/m2 h (5 kDa) and peptide transmission of 58.20% (10 kDa) and 67.34% (5 kDa). Meanwhile, for two-stage (10/5 kDa) UF membrane, the best parameters were observed at a flow rate of 23 mL/min, TMP of 1.5 bar, and pH of 2, with permeate flux of 69.85 L/m2 h and peptide transmission of 79.13%. Fractionation of MPH with two-stage UF membrane was observed to be better at producing a low molecular weight of peptide compared to single UF membrane. In conclusion, it was possible to produce permeate flux with a high amount of low molecular weight of peptide by controlling the operating parameters with the suitable configuration membrane.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Institute of Plantation Studies
DOI Number: https://doi.org/10.3390/pr9040610
Publisher: MDPI AG
Keywords: Microalgae protein hydrolysate; Peptide; Cross-flow ultrafiltration membrane; Permeate flux; Peptide transmission
Depositing User: Ms. Ainur Aqidah Hamzah
Date Deposited: 02 Aug 2022 07:09
Last Modified: 02 Aug 2022 07:09
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/pr9040610
URI: http://psasir.upm.edu.my/id/eprint/95556
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