Shoot induction of selected Dendrocalamus asper genotype

Dendrocalamus.asper is well-known for its rapid growth, thick-walled characteristics and can be an alternative for timber in the future. Micropropagation is one of the appropriate strategies to obtain reasonable amount of bamboo supply from small quantity and independent from seasonal growth. This study was conducted to determine the optimum concentration of BAP in shoot induction of selected D.asper genotype. Murashige and Skoog basal medium was used for culture establishment and shoot induction. As for plant growth regulator, the cytokinin used was N6-benzyladenine (BAP) with four different concentrations which were (0, 1, 3, and 5) mg/l on micropropagation of D. asper genotype was used for shoot induction purpose. Zero mg/l (without BAP) was used as control medium. Each treatment contained 32 replicates and was observed two weeks after being transferred from initial medium to cytokinin supplemented medium during the subculture periods. The results from this study indicated that best sterilization treatment used for D. asper shoot induction is in 0.1% HgCl2 solution for 10 minutes and best concentration of cytokinin length of shoot is 5.0 mg/l of BAP respectively. As for number of shoot, it required higher concentration than 5.0 mg/l to give better response in new shoot production.

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