Siderophore production activity of rhizospheric& lactic acid bacteria for papaya growth promotion

Siderophore produced by microbial cells helps the plant to acquire iron from the surrounding. Iron uptake able to promote plant growth at the same time, acts antagonistically against pathogen by limiting iron availability. Preliminary study on Lactic Acid Bacteria (LAB) showed its potential against papaya dieback disease which was caused by causative pathogen (Erwinia mallotivora). The ability of LAB to produce siderophore was investigated in order to enhance their properties as biocontrol and plant growth promoter. The microbes present in rhizosphere of healthy papaya plant were screened for LAB that capable of producing siderophore using Chrome Azurol S (CAS) agar. CAS agar effectively differentiates bacteria capable of producing siderophore with non producer with simple colour changes to form orangy colony zone. Out of twelve rhizospheric and endophytic bacteria grown in selective LAB media, three isolates were tested positive for siderophore production. The characteristics of the positive isolates were confirmed by biochemical test for LAB and their species were identified using 16S rRNA sequencing.

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