Citation
Mohd Rahim, Syarifah and Salleh, Amizatul Aini and Bakrin, Ikmal Hisyam and Tengku Jamaluddin, Tengku Zetty Maztura and Masri, Siti Norbaya
(2020)
Molecular detection of Cryptococcus from formalin-fixed
paraffin-embedded tissue sample - a noncultural approach
for diagnosis of primary pulmonary cryptococcosis.
Malaysian Journal of Medicine and Health Sciences, 16 (spec. 1).
pp. 47-49.
ISSN 1675-8544; ESSN: 2636-9346
Abstract
Despite pulmonary cryptococcosis showing good response to antifungal treatment, making accurate diagnosis in a timely manner remains a challenge. Tissue for fungal culture is less sensitive, nonspecific, and time-consuming to allow for prompt diagnosis. We herein report a case of 31-year-old woman with no known medical illness but presented with history of cough and haemoptysis for two months. The chest X-ray and bronchoscopy revealed left lung mass. While tuberculosis showed negative result and no growth observed in the tissue culture, the histopathological examination (HPE) finding was suggestive of fungal infection. The formalin-fixed paraffin-embedded (FFPE) tissue was sent for molecular testing, which revealed Cryptococcus neoformans. This report emphasises on the advantages and limitations of polymerase chain reaction (PCR) as an alternative method to confirm the diagnosis in cases of culture-negative fungal infection.
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