The effect of Pseudomonas spp. and UPMB10 and different rate of urea fertilizer on protein content and growth of leaf mustard

Green mustard (Brassica sp.) is a type of plant in the mustard category of the family Cruciferae. This plant is one out of five types of broad leaf vegetables commonly grown in Malaysia every year. Nitrogen is one the most yield limiting nutrients for most crop production in the world except for legumes, which have the ability to fix their own N. A study on the effect of Pseudomonas spp. and UPMB10 and different rate Urea fertilizer on protein content and growth of green mustard was conducted. The main objective is to study if the rate of nitrogen fertilizer and used of bacterial inoculum can increase protein content and growth of the plant. There were 10 treatments including two control treatments with four replications arranged in a Completely Randomized Design (CRD). The treatments were; T1 (Not inoculated + 0 g Urea), T2 (Not inoculated + 0 g Urea), T3 [Inoculated with UPMB-10 (Bacillus sphaericus) + 0 g Urea], T4 [Inoculated with UPMB-10 ( Bacillus sphaericus )+0.49 g Urea], T5 [Inoculated with UPMB-10 (Bacillus sphaericus) + 0.98 g Urea], T6 [Inoculated with UPMB-10 (Bacillus sphaericus) + 2.94 g Urea], T7 (Inoculated with Pseudomonas spp. + 0 g Urea), T8 (Inoculated with Pseudomonas spp. + 0.49 g Urea), T9 (Inoculated with Pseudomonas spp. + 0.98 g Urea), T10 (Inoculated with Pseudomonas spp. + 2.94 g Urea). The parameters involved in this project were the plant fresh and dry weight, chlorophyll content, plant height, nutrient composition and protein content. The results indicate that used of bacteria utilization by injection inoculation method significantly improved the growth of the plant. The fresh weight of the plant increase by 20% , dry weight increase by 14%, plant height increase by 22% and chlorophyll content of the plant increase by 9% as compared to the control treatment. While rate of Urea (N) fertilizer improved the nutrient composition (N- 19%, P- 3%, K- 15%) and protein content of the plant increase by 30%.

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