Citation
Abstract
Free biocatalyst such as lipase cannot be recovered and reused; and is extremely unstable in harsh reaction condition. Present study aimed to synthesize hierarchical ordered macro-microporous ZIF-8 structures (MAC-ZIF-8) for encapsulation of nano-Burkholderia cepacia lipase (nano BCL). The synthesized MAC-ZIF-8 has good crystallinity, thermal stability and high surface area which made it suitable for immobilization of nano-BCL. The MAC-ZIF-8 immobilized nano-BCL (nano-BCL@MAC-ZIF-8) demonstrated high enzyme activity (4.3778 U) and can be recovered and reused for consecutive hydrolysis reaction. It is found that the nano-BCL is well encapsulated with the macropores of the MAC-ZIF-8 structures and they are able to preserve their native configuration with high affinity for substrates (lower Km of 1.068 mM as compared to that of nano-BCL@ZIF-8). The nano-BCL@MAC-ZIF-8 can also be used to fabricate electrochemical biosensors for detection of nitrogenous diphenyl ether pesticides (nitrofen) in real food systems (apricots). The fabricated biosensors demonstrated wide linear range (0−114µM), low limit of detection (0.46µM) and good recovery rate. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, low cost, and better linear range.
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Food Science and Technology |
DOI Number: | https://doi.org/10.1016/j.snb.2020.128477 |
Publisher: | Elsevier |
Keywords: | Burkholderia cepacia lipase; Lipase nanogelZIF-8; Immobilization; Electrochemical detection; Nitrogenous diphenyl ether pesticides |
Depositing User: | Ms. Nuraida Ibrahim |
Date Deposited: | 03 Sep 2021 23:28 |
Last Modified: | 03 Sep 2021 23:28 |
Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.snb.2020.128477 |
URI: | http://psasir.upm.edu.my/id/eprint/89177 |
Statistic Details: | View Download Statistic |
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