Citation
Loke, Wai Keong and Mohd Saud, Halimi
(2020)
Electrotransformation on selected plant growth-promoting Rhizobacteria (PGPR) and Chromobacterium violaceum.
Journal of Agrobiotechnology, 11 (1).
63 - 69.
ISSN 2180-1983
Abstract
Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Agriculture |
| DOI Number: | https://doi.org/10.37231/jab.2020.11.1.214 |
| Publisher: | Penerbit Universiti Sultan Zainal Abidin |
| Keywords: | Electrotransformation; Plant Growth-Promoting Rhizobacteria (PGPR); Chromobacterium violaceum; Cosmid vector pLAFR1 |
| Depositing User: | Ms. Nuraida Ibrahim |
| Date Deposited: | 03 Nov 2021 03:53 |
| Last Modified: | 03 Nov 2021 03:53 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.37231/jab.2020.11.1.214 |
| URI: | http://psasir.upm.edu.my/id/eprint/88779 |
| Statistic Details: | View Download Statistic |
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