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Nutritional composition, phytochemicals and acute toxicity of herbal mixture (lemon, apple cider, garlic, ginger and honey) in Zebrafish embryo and Wistar rat


Citation

Abu Bakar Sajak, Azliana and Azlan, Azrina and Abas, Faridah and Hamzah, Hazilawati (2020) Nutritional composition, phytochemicals and acute toxicity of herbal mixture (lemon, apple cider, garlic, ginger and honey) in Zebrafish embryo and Wistar rat. Food Research, 4 (suppl.1). 196 - 204. ISSN 2550-2166

Abstract

This study was aimed to provide the reference frame for the safe dose design of polyphenol-rich herbal mixture, which consist of lemon, apple cider, garlic, ginger and honey (PRM) for the future efficacy study. Prior to this, the nutritional composition was first conducted and the identification of metabolites that present in PRM was determined using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The acute toxicity of the PRM was then evaluated in zebrafish embryo and Wistar rats following The Organisation for Economic Co-operation and Development (OECD) guidelines. The PRM nutritional composition and sugar profile showed it was high in carbohydrate, ash and protein and the main sugar is fructose. It also contains metabolites such as fructosefuranose, lactic acid, ascorbic acid, acetic acid, cycloalliin, pyruvate, 5- hydroxymethylfurane, α- and β-glucose. From the zebrafish embryo acute toxicity result, the lethal concentration, LC50 of PRM was at 487.50 μg/mL. Meanwhile, in Wistar rats’ model, no lethality was observed in the group treated with PRM at the end of the study (14 days). No changes were also observed from the behavioural and appetite as well as the biochemical parameters (AST, ALT, total cholesterol, triglyceride, LDL, HDL, total protein and creatinine) of the treated group. Therefore, the safe dose for PRM can be up to 2000 mg/kg b.w. in Wistar rats and below 487.50 μg/mL in zebrafish embryo model.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.26656/fr.2017.4(S1).S11
Publisher: Rynnye Lyan Resources
Keywords: Toxicity; Nuclear magnetic resonance; Animal model; Proximate analysis
Depositing User: Mohamad Jefri Mohamed Fauzi
Date Deposited: 07 Jan 2022 08:41
Last Modified: 07 Jan 2022 08:41
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.26656/fr.2017.4(S1).S11
URI: http://psasir.upm.edu.my/id/eprint/86945
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