Differential cell counts in 5 commercial ornamental fishes

The aim of this study was to determine the percentage of differential types of leukocytes in commercial ornamental fish (Green Tiger Barb - Puntigrus tetrazona, Rosy Tetra - Hyphessobrycon rosaceus, Rosy Barb - Pethia conchonius, Red Eye Tetra - Moenkhausia sanctaefilomenae and Red Zebra Danio - Danio rerio) and G:L (granulocyte to lymphocyte) ratio as stress index of these species. This study examined 20 samples (four specimens for each species) from the controlled conditions in good health condition. The leukocytes stained by May-Grunwald Giemsa solution and were classified to four types; lymphocytes, granulocytes, monocytes and thrombocytes. Lymphocytes were the most abundant leukocytes found in the peripheral blood of all tested species, in which the lowest percentage lymphocytes was recorded in Rosy Tetra (81.74±3.27) while the highest value was Red Eye Tetra (94.57±1.32). The G:L ratio of the tested species were lower than 0.5, where Rosy Tetra recorded the highest G:L ratio among the tested (0.120±0.021), meanwhile Red Eye Tetra was the lowest (0.007±0.003). The current result provides the reference values and background for the future experiments. Any increment from the G:L ratio of the respective species reported in this study can be an indication of stress.

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