In vitro rooting of Clinacanthus nutans as affected by combination of different Murashige & Skoog salt and IBA concentrations

Clinacanthus nutans is a medicinal plant widely grown in tropical Asia and locally known “Belalai Gajah” or Sabah Snake Grass. Low reproductive capacity and non-uniform plant materials of Clinacanthus nutans propagated by stem cutting had brought to a study on the best combination of MS salt and IBA concentrations for rooting of Clinacanthus nutans explant under in vitro condition. This experiment was carried out at In Vitro Laboratory, Department of Agriculture, Universiti Putra Malaysia and conducted using Completely Randomized Design (CRD). Medium with full strength and half strength of Murashige and Skoog (1962) medium formulation were supplemented with indole butyric acid (IBA) at 0, 0.1, 1.0, 2.5 and 5.0 mg/L respectively. Shoot of axenic cultures of Clinacanthus nutans with a size of 2.5 cm was cultured in these treatments with ten replicates each. It has been observed that medium with half strength MS salt supplemented with 0.1 mg/L IBA gave good rooting of Clinacanthus nutans as it produced 90% of explant rooted with two roots formed per explant and a reasonable root length of 1.38 cm. This treatment gives an economical benefit by reducing the cost of planting material production.

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