Citation
Lai, Shu Ying
(2015)
Determination of lipase and amylase activities from wood decay fungi.
[Project Paper Report]
Abstract
Fungal enzymes have long history of usage in various industries. Lipase and amylase
are two of the most commercialized enzymes to date. Wood decay fungi are
promising source of amylase and lipase. A number of fungi had been isolated from
decayed tree bark. Isolates were screened with minimal media supplemented with
specific carbon source, starch and tween 80 respectively.. Starch was used for
screening of amylase producing fungi while tween 80 was used for screening of
lipase producing fungi. Two amylase producing fungi identified to be Aspergillus sp.
and Fusarium sp. were isolated. For lipase producing fungi, Cladosporium sp. and
Aspergilus sp. were isolated. Fungal isolates were analyzed for its amylase and
lipase activity. Fusarium sp. has highest amylase activity of 53.69U/ml while
Cladosporium sp. reported highest lipase activity of 503.02 U/ml. Growth rate
analysis was done to further verify the amylolytic and lypolytic capability of isolates.
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