UPM Institutional Repository

Isolation and characterization of bacteriophage from crab


Mohamad Safiee, Amira Wahida (2015) Isolation and characterization of bacteriophage from crab. [Project Paper Report]


This project was carried out to isolate, purify and characterize the bacteriophage from the crab (Callinectus sapidus) also known as blue crab. The crab was obtained freshly from Jetty Wak Sempo, Bagan Lalang, Sepang. Seven bacteria was isolated from the crab and labelled as A, B, C, D, E, F and G were used as a screening agent for the isolation of the bacteriophage. Besides, another 5 species of known bacteria were used for bacteriophage isolation. The bacteria were Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus and Staphylococcus aureus. All 12 species of bacteria were used but only bacteria C was infected by the bacteriophage. The bacteriophage was labelled as C-CC. The bacteriophage infected bacteria was identified by gram staining and biochemical tests. Based on the gram staining, the bacterium is a gram negative bacterium with coccobacillus shape. Then, the isolated bacteriophage was characterized based on their protein, genomic, biology and physical characteristics. At the end of the study, the isolated bacteriophage was confirmed classified as DNA-containing bacteriophage with lytic life cycle. The bacteriophage could be as used in the phage therapy against the bacterial contamination.

Download File

[img] Text
FBSB 2015 93 - IR.pdf

Download (1MB)

Additional Metadata

Item Type: Project Paper Report
Call Number: FBSB 2015 93
Chairman Supervisor: Prof. Dr. Tan Wen Siang
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 27 Dec 2021 07:48
Last Modified: 27 Dec 2021 07:48
URI: http://psasir.upm.edu.my/id/eprint/85104
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item