Isolation and analysis of the rhla and rhlb from rhamnolipid producing bacterium Pseudomonas aeruginosa strains S5

Rhamnolipids are biotechnologically important biosurfactants, find applications in cosmetic and pharmaceutical industry, agriculture, environmental application and in bioremediation activities. Pseudomonas aeruginosa is one of the microbes that can produce rhamnolipids. The aim of this study is to test the rhamnolipids production of our P. aeruginosa strain and to analyze the genes required for the rhamnolipids biosynthesis. Based on the qualitative test on cetyltrimethyl ammonium bromide agar, P. aeruginosa strain S5 formed a blue halo around the culture which showed that the bacterium is a rhamnolipids producer. The emulsification index value of the rhamnolipids produced was 41%. Genes involved in rhamnolipids biosynthesis in P. aeruginosa strain S5; rhlA coding for rhamnosyltransferase 1 subunit A, and rhlB, coding for rhamnosyltransferase 1 subunit B were amplified, sequenced and analyzed. The sequencing results showed that the size of rhlA and rhlB, genes are 888 bp and 1281 bp, which corresponded to 295 and 426 amino acids protein, respectively. The genes sequence that were identified and analyzed could facilitate the understanding on the rhamnolipids biosynthesis in P. aeruginosa strain S5, hence the information can be further used in genetic modulation in order to produce natural biosurfactant in the economic yet safer manner which meets industrial and ecological prospect.

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