The influence of serum‐supplemented culture media in a transwell migration assay

Citation

Omar Zaki, Siti Sarah and Kanesan, Livashini and Leong, Ming Yeh Danielle and Vidyadaran, Sharmili (2019) The influence of serum‐supplemented culture media in a transwell migration assay. Cell Biology International, 43 (10). pp. 1201-1204. ISSN 1065-6995; ESSN: 1095-8355

Abstract

Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Medicine and Health Science
DOI Number:	https://doi.org/10.1002/cbin.11122
Publisher:	Wiley
Keywords:	BV2 microglia; Chemoattractant; Serum; Transwell migration assay
Depositing User:	Mr. Sazali Mohamad
Date Deposited:	16 Oct 2020 20:20
Last Modified:	16 Oct 2020 20:20
Altmetrics:	http://www.altmetric.com/details.php?domain=psair.upmedu.my&doi=10.1002/cbin.11122
URI:	http://psasir.upm.edu.my/id/eprint/82524
Statistic Details:	View Download Statistic

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