UPM Institutional Repository

Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology


Citation

N., Shobana and Samrot, Anthony V. and Subbiah, Suresh Kumar and G., Narendrakumar (2019) Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology. Journal of Cluster Science, 30. pp. 607-620. ISSN 1040-7278; ESSN: 1572-8862

Abstract

Chitosanases is a class of enzymes which hydrolyse chitosan, a natural biopolymer consisting of d-glucosamine in various degrees. In this study, chitosanase producing Bacillus sp. was isolated from soil sample. Chitosanase production was optimized using response surface methodology and the produced chitosanase was characterized. The crude enzyme was found to possess antibacterial and antifungal activity. Chitosanase enzyme was used for trimming chitosan based polymeric nanoparticles produced using sodium trimetaphosphate chelator. Chitosanase enzyme was also utilized for synthesis of silver nanoparticles which were then characterized by UV–Vis, FTIR, SEM, TEM and AFM. The produced nanoparticles were checked for antibacterial and antifungal activity.


Download File

[img] Text (Abstract)
Production, optimization and characterisation of chitosanase of Bacillus sp. and its applications in nanotechnology.pdf
Download (97kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s10876-019-01520-z
Publisher: Springer
Keywords: Chitosanase; Optimization; Characterization; Silver nanoparticles production
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 28 Aug 2020 01:16
Last Modified: 28 Aug 2020 01:16
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10876-019-01520-z
URI: http://psasir.upm.edu.my/id/eprint/81851
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item