Citation
Abstract
This study investigates the ultrasound-assisted extraction of flavonoids from Malaysian cocoa shell extracts, and optimization using response surface methodology. There are three variables involved in this study, namely: ethanol concentration (70⁻90 v/v %), temperature (45⁻65 °C), and ultrasound irradiation time (30⁻60 min). All of the data were collected and analyzed for variance (ANOVA). The coefficient of determination (R²) and the model was significant in interaction between all variables (98% and p < 0.0001, respectively). In addition, the lack of fit test for the model was not of significance, with p > 0.0684. The ethanol concentration, temperature, and ultrasound irradiation time that yielded the maximum value of the total flavonoid content (TFC; 7.47 mg RE/g dried weight (DW)) was 80%, 55 °C, and 45 min, respectively. The optimum value from the validation of the experimental TFC was 7.23 ± 0.15 mg of rutin, equivalent per gram of extract with ethanol concentration, temperature, and ultrasound irradiation time values of 74.20%, 49.99 °C, and 42.82 min, respectively. While the modelled equation fits the data, the T-test is not significant, suggesting that the experimental values agree with those predicted by the response surface methodology models.
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Official URL or Download Paper: https://pubmed.ncbi.nlm.nih.gov/30781448/
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Additional Metadata
Item Type: | Article |
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Divisions: | Faculty of Agriculture Faculty of Biotechnology and Biomolecular Sciences Halal Products Research Institute |
DOI Number: | https://doi.org/10.3390/molecules24040711 |
Publisher: | Multidisciplinary Digital Publishing Institute |
Keywords: | Cocoa shell; Flavonoids; Response surface methodology; Ultrasound-assisted extraction |
Depositing User: | Ms. Nida Hidayati Ghazali |
Date Deposited: | 29 Oct 2020 19:40 |
Last Modified: | 29 Oct 2020 19:40 |
Altmetrics: | https://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules24040711 |
URI: | http://psasir.upm.edu.my/id/eprint/81560 |
Statistic Details: | View Download Statistic |
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