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Metabolites and biological activities of Phoenix dactylifera L. pulp and seeds: a comparative MS and NMR based metabolomics approach


Abdul Hamid, Nur Ashikin and Abas, Faridah and Ismail, Intan Safinar and Chau, Ling Tham and Maulidiani, M. and Mediani, Ahmed and Swarup, Sanjay and Umashankar, Shivshankar and Zolkeflee, Nur Khaleeda Zulaikha (2019) Metabolites and biological activities of Phoenix dactylifera L. pulp and seeds: a comparative MS and NMR based metabolomics approach. Phytochemistry Letters, 31. pp. 20-32. ISSN 1874-3900; ESSN: 1876-7486


Phoenix dactylifera L. (date palm) is an essential agricultural crop in many arid regions of the world and has been used in traditional medicine to treat illnesses. Extracts of P. dactylifera are reported to possess valuable pharmacological attributes. This study aims to evaluate the metabolite profiles of date pulp and seeds utilizing 1H nuclear magnetic resonance (NMR) and ultra-high performance liquid chromatography mass spectrometry (UHPLC-MS) techniques. The nitric oxide (NO) inhibitory activity of extracts were assessed using stimulated RAW 264.7 cells along with the NO and DPPH scavenging activities, and total phenolic contents (TPC). Multivariate data analysis (MVDA) was used to examine the similarities and variation between samples of date palms. Principal component analysis (PCA) score plots showed distinct clustering of date seeds and pulp. Examination of the S-plot analysis revealed that ascorbic acid, catechin and glucose were the metabolites that significantly discriminated the date pulp group from the seed group. The seeds of Deglet dates demonstrated significantly higher NO inhibitory and DPPH scavenging activities, with IC50 values of 107.99 μg/mL and 12.58 μg/mL, respectively. The metabolites that prominently contributed to the observed bioactivities included catechin, ascorbic acid and serine. The proposed metabolic pathway indicated that higher amounts of several metabolites are present in date seeds than in date pulp. The presented data suggest possible applications of seeds and pulp of P. dactylifera that may increase the productivity of this fruit crop.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Faculty of Medicine and Health Science
Faculty of Science
DOI Number: https://doi.org/10.1016/j.phytol.2019.03.004
Publisher: Elsevier
Keywords: Phoenix dactylifera; Deglet dates; Metabolomics; Date seeds; Date pulps; Nitric oxide
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 14 Jun 2021 14:24
Last Modified: 14 Jun 2021 14:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.phytol.2019.03.004
URI: http://psasir.upm.edu.my/id/eprint/81337
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