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Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways,


Citation

Su, Chern Foo and Md. Yusoff, Fatimah and Umar Imam, Mustapha and Jhi, Biau Foo and Ismail, Nor Sharina and Azmi, Nur Hanisah and Yin, Sim Tor and Khong, Nicholas Mun Hoe and Ismail, Maznah (2019) Increased fucoxanthin in Chaetoceros calcitrans extract exacerbates apoptosis in liver cancer cells via multiple targeted cellular pathways,. Biotechnology Reports, 21. art. no. e00296. pp. 1-10. ISSN 2215-017X

Abstract

In this study, anti-proliferative effects of C. calcitrans extract and its fucoxanthin rich fraction (FxRF) were assessed on human liver HepG2 cancer cell line. Efficacy from each extract was determined by cytotoxicity assay, morphological observation, and cell cycle analysis. Mechanisms of action observed were evaluated using multiplex gene expression analysis. Results showed that CME and FxRF induced cytotoxicity to HepG2 cells in a dose and time-dependent manner. FxRF (IC50: 18.89 μg.mL−1) was found to be significantly more potent than CME (IC50: 87.5 μg.mL−1) (p < 0.05). Gene expression studies revealed that anti-proliferative effects in treated cells by C. calcitrans extracts were mediated partly through the modulation of numerous genes involved in cell signaling (AKT1, ERK1/2, JNK), apoptosis (BAX, BID, Bcl-2, APAF, CYCS) and oxidative stress (SOD1, SOD2, CAT). Overall, C. calcitrans extracts demonstrated effective intervention against HepG2 cancer cells where enhanced apoptotic activities were observed with increased fucoxanthin content.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.1016/j.btre.2018.e00296
Publisher: Elsevier BV
Keywords: Chaetoceros calcitrans; Microalgae; Fucoxanthin; Rich fraction; Apoptosis; Gene expression
Depositing User: Azhar Abdul Rahman
Date Deposited: 02 Oct 2020 08:53
Last Modified: 02 Oct 2020 08:55
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.btre.2018.e00296
URI: http://psasir.upm.edu.my/id/eprint/80234
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