UPM Institutional Repository

Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant Boer goats


Citation

Tanko, Polycarp Nwunuji and Mohd Yusoff, Sabri and Emikpe, Benjamin Obukowho and Bejo, Siti Khairani and Salisi, Sharom (2019) Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant Boer goats. Journal of Immunoassay and Immunochemistry, 40 (4). pp. 419-438. ISSN 1532-1819; ESSN: 1532-4230

Abstract

Brucellosis constitutes an infectious re-emerging zoonosis. Spread of diseases could be exacerbated by stress-induced immunosuppression. This study evaluated relationship between Brucella melitensis infection, shedding dynamics, dexamethasone-induced stress, pathological alterations and resveratrol ameliorative effects in goats. Twelve nonpregnant goats were divided into four groups A, B, C, and D of three animals each. Groups A and B were administered 107 CFU/mL of B. melitensis ocularly, 21 days prior to 7 days consecutive administration of dexamethasone (2 mg/kg). Group A was further administered resveratrol (5 mg/kg) intravenously for 5 consecutive days from day 31 post B. melitensis inoculation. Group C was administered similar dose of B. melitensis while group D was inoculated normal saline. Blood, nasal, ocular, and vaginal swabs were collected at intervals for analysis. The does were sacrificed at day 42 post inoculation (pi). Tissues were collected for tissue bacterial load determination, histopathology, and immunohistochemistry. Dexamethasone administration from day 21 pi increased the frequency in the shedding dynamics, tissue bacterial load, pathological alterations (frequency of microgranuloma and intensity of immunostaining) in group B while 5 days treatment with resveratrol following dexamethasone administration significantly reduced tissue bacterial load, decline in shedding dynamics, and ameliorate damage by dexamethasone administration/B. melitensis infection.


Download File

[img] Text
Effects of resveratrol on shedding and pathological dynamics in experimental B. melitensis infection in dexamethasone-treated nonpregnant Boer goats.pdf

Download (9kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1080/15321819.2019.1620766
Publisher: Taylor and Francis
Keywords: Dexamethasone-induced stress; Immunohistochemistry; Pathological alteration; Shedding dynamics
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 14 Aug 2021 23:43
Last Modified: 14 Aug 2021 23:43
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/15321819.2019.1620766
URI: http://psasir.upm.edu.my/id/eprint/79618
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item