UPM Institutional Repository

Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid


Citation

Chin, Chee Keong and Lee, Ze Hong and Mubbarakh, Safiah Ahmad and James Antony, Jessica Jeyanthi and Chew, Bee Lynn and Subramaniam, Sreeramanan (2019) Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid. Biocatalysis and Agricultural Biotechnology, 22 (1). pp. 1-8. ISSN 1878-8181

Abstract

Protocorm-like bodies (PLBs) of Dendrobium orchids are emerging as a potential source of valuable secondary metabolites. This study examined the effect of four additives namely 1-naphthaleneacetic acid (NAA), kinetin, thidiazuron (TDZ), and activated charcoal (AC) used in culture medium on genetic variability in PLBs of Dendrobium Sabin Blue. Nine (9) ISSR primers and eleven (11) DAMD primers were used to assess the genetic variability of PLBs that were subcultured over a period of two years. We confirmed that the use of kinetin in culture medium for two years resulted in the highest rate of somaclonal variation in PLBs. On the other hand, TDZ and activated charcoal registered the lowest genetic variability in PLBs. The findings of this study suggest the importance of selecting additives used in the culture medium to maintain stable genetic lines of PLBs. We recommend that the assessment of somaclonal variations should be performed for long term maintenance of tissue cultures.


Download File

[img] Text
Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid.pdf

Download (56kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture and Food Sciences
DOI Number: https://doi.org/10.1016/j.bcab.2019.101426
Publisher: Elsevier
Keywords: Orchid; Protocorm-like bodies; Somaclonal variations; DNA markers
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 14 Aug 2021 11:25
Last Modified: 14 Aug 2021 11:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.bcab.2019.101426
URI: http://psasir.upm.edu.my/id/eprint/79611
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item