Plasma bovine growth hormone (bGH) in buffaloes and its associations with selected phenotypes

Forty buffaloes with different phenotypes were used in the study. The phenotypes were the breed, sex, current body weight, age, birth weight and age at puberty. Blood samples were collected from the coccygeal vein into ethylenediamine tetraacetic acid (EDTA) tubes. Plasma was harvested after centrifugation at 3000 rpm for 15 minutes. Concentrations of plasma bovine growth hormone (bGH) were determined by quantitative sandwich ELISA using commercial kits from Novateinbio Bovine Growth Hormone ELISA Kit. The data on selected phenotypes were collected from the farm records retrospectively between 2006 and 2015. Breeds wise, there was no significant difference (p>0.05) among Swamp and Murrah Cross in plasma bGH concentration. There was also no significant difference (p>0.05) among female and male buffaloes in plasma bGH concentration. In age groups, there was no significant difference (p>0.05) among young, adult and old buffaloes in plasma bGH concentration. The correlation coefficient between (r) between plasma bGH concentration, body weight, age, birth weight and age at puberty were tested which plasma bGH concentration was negatively correlated with body weight (r = -0.069), age (r = -0.111), birth weight(r = -0.141) and age at puberty (r = -0.062). Thus, in this study, plasma bGH concentration did not showed any association with selected phenotypes.

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