Detection of Salmonella and E.coli in Edible Bird's Nest ranched in housing system

The swiftlet industry in Malaysia is growing very fast due to the high demand for Edible Bird’s Nest (EBN) . The presence of bacteria may produce low quality of EBN that can lead to economic loss and may cause food-borne diseases. The aim of this study was to detect the presence of Salmonella and E.coli in EBN and guano of edible-nest swiftlets ranched in housing system and to enumerate the total number of bacteria and coliform in EBN. In this study, a total of 64 guano and nest swab samples were collected from three bird houses in Terengganu. The samples were pre-enriched and enriched before culturing on Brilliant Green Agar (BGA) and Xylose Lysine Deoxycholate (XLD) for isolation of Salmonella and propagated in nutrient broth before culture on Eosin Methylene Blue Agar (EMBA) for isolation of E.coli. The isolation of the bacteria was carried out in Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, UPM. Standard Plate Count (SPC) and Coliform count using 3M® Petrifilm were done. No Salmonella was isolated in EBN and the prevalence of Salmonella in guano was 12.5% as compared to E. coli in EBN at 3.13% and 68.75% in guano sample. The average SPC of EBNs was 3.2 x 105 CFU per gram and CPC was ≤ 100 CFU per gram . E.coli in EBNs swab were lower than in guano samples. Hence, the absence of Salmonella and low number of E.coli in EBN is a good news for the growing swiftlet industry.

Text FPV 2016 63 IR.pdf Download (1MB)

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