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Isolation, screening and characterization of biosurfactant producing microorganisms


Halim, Siti Zulaikha Suryati (2015) Isolation, screening and characterization of biosurfactant producing microorganisms. [Project Paper Report]


Biosurfactants are amphiphilic compounds comprising of a non-polar (hydrophobic) group and a polar (hydrophilic) group which are produced by microorganisms. Biosurfactants play an important role in bioremediation processes due to their effectiveness as dispersion and bioremediation agents as well as their environmentally characteristics, such as low toxicity and high biodegradability than chemical surfactants. However, the high production cost of biosurfactants is a major drawback in the industry. Used cooking oil is one of the alternative cheap substrate for biosurfactant production. The aim of this study is to isolate, screen and characterize biosurfactant-producing bacteria from the sources of used cooking oil, contaminated soil, and palm oil mill effluent (POME). Four strains which designated as HIO 1, HIP 1, HIP 2 and HIP 3 were able to produce biosurfactant from used cooking oil. Identification using 16S rRNA reveals that HIP 1 and HIP 2 are Lysinibacillus strain while HIP 3 and HIO 1 are strains of Bacillus. HIP 3 has the biggest oil displacement area with 92.12 cm2 when tested using oil spreading assay. All the bacterial strains were identified as the biosurfactants producer.

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Additional Metadata

Item Type: Project Paper Report
Call Number: FBSB 2015 43
Chairman Supervisor: Professor Dr. Suraini Abd. Aziz
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Depositing User: Ms. Nur Faseha Mohd Kadim
Date Deposited: 26 Jun 2020 00:36
Last Modified: 26 Jun 2020 00:36
URI: http://psasir.upm.edu.my/id/eprint/78197
Statistic Details: View Download Statistic

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