Resistance capability of indigenous and non-indigenous bacteria species on 3,4-dichoroaniline

3,4- dichloroaniline (3,4-DCA) is commonly be used in the chemical industry and it is also a by-product of aniline-derived herbicides such as diuron, linuron and propanil. However, 3,4-DCA exhibits higher toxicity than its parent compound and persistent in soils, water and groundwater. The aim of study was to investigate three TBT-resistant bacteria which are Klebsiella sp., Acinetobacter sp., Citrobacter sp. to evaluate their resistancy towards 3,4-DCA. Pseudomonas sp. isolated from non-TBT-polluted area was used as a positive control. Respiratory inhibitory activity of 3,4-DCA on three bacteria strains were assessed by employing MTT-bioassay. Results showed, Citrobacter sp. is the most tolerable bacteria amongst three strains with the IC50 value of 7.83 mg/L followed by Acinetobacter sp. (7.55 mg/L), Klebsiella sp. (7.09 mg/L) and Pseudomonas sp. (7.02 mg/L). Due to that, potential TBT-degrading bacteria, Klebsiella sp. is not preferable bacteria to degrade or utilize 3,4-DCA mainly because of the structure and resistancy bacteria itself. Thus, future studies are recommended in order to remediate this highly persistant compound in solving environmental problems associated contamination.

Text (Abstract) i-SIMBIOMAS 2016 62.pdf Download (220kB)

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