Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model

Citation

Z. Z., Nurfarizatul and N. K. P., Diyana and F. M., Amirah and Mohd Yaminudin, Nur Jasmin and Karim, M. and Md Yasin, Ina Salwany (2016) Molecular characterization of three Vibrio species and study its pathogenic potential in a gnotobiotic Artemia model. In: Malaysia International Biology Symposium 2016, 26-27 Oct. 2016, PICC, Putrajaya, Malaysia. (p. 45).

Abstract

Vibriosis remains the main problem in aquaculture industry caused by bacteria from the genus Vibrio. This study was developed to identify and differentiate between the three Vibrio species which are Vibrio harveyi, V. alginolyticus and V. parahaemolyticus by PCR-outer membrane protein (ompW) gene. Three specific bands with size of 643 bp were produced and sequence analysis of three Vibrios used in this study showed high similarities of V. harveyi, V. alginolyticus and V. parahaemolyticus with published sequences, respectively. Thus, ompW gene can be used as phylomarker in differentiating Vibrio isolates. The effect of pathogenicity of local Vibrios strains with different concentrations on host-microbe interaction has been studied in the gnotobitic Artemia. After challenged, investigation revealed that maximum mortality of Artemia was observed at the concentration of 107 CFU/mL with V. parahaemolyticus have a highest mortality rate.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Agriculture Institute of Bioscience
Publisher:	Department of Biology, Faculty of Science, Universiti Putra Malaysia
Keywords:	Vibrio species; ompW; Gnotobiotic Artemia
Depositing User:	Nabilah Mustapa
Date Deposited:	15 Apr 2020 16:43
Last Modified:	15 Apr 2020 16:43
URI:	http://psasir.upm.edu.my/id/eprint/77504
Statistic Details:	View Download Statistic

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