Citation
Md Hatta, Muhammad Asyraf and Johnson, Ryan and Matny, Oadi and Smedley, Mark A. and Yu, Guotai and Chakraborty, Soma and Bhatt, Dhara and Xia, Xiaodi and Arora, Sanu and Steuernagel, Burkhard and Richardson, Terese and Mago, Rohit and Lagudah, Evans S. and Patron, Nicola and Ayliffe, Mick and Rouse, Matthew N. and Harwood, Wendy A. and Periyannan, Sambasivam K. and Steffenson, Brian J. and Wulff, Brande B. H.
(2018)
Host range expansion of wheat stem rust resistance genes into barley.
In: Joint Symposium of the 8th International Agriculture Congress 2018 and 6th International Symposium for Food & Agriculture 2018 (8th IAC – 6th ISFA 2018), 13-15 Nov. 2018, Auditorium Rashdan Baba, TNCPI Building, Universiti Putra Malaysia. (pp. 100-103).
Abstract
In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re-emerged as a major threat to wheat and barley cultivation in Africa and Europe. In contrast to wheat with 82 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only seven resistance genes genetically identified. Of these, only one locus consisting of two genes is effective against Ug99, a strain of Pgt which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against Ug99. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium-mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.
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