Citation
Huwaidi, Alaa Hassan Ibrahim Ahmed
(2018)
Identification of mycosporinelike amino acids and 3-dehydroquinate synthase gene expression in UV radiation-induced Deinococcus radiodurans R1.
Masters thesis, Universiti Putra Malaysia.
Abstract
Deinococcus radiodurans R1 is a well-known heterotrofic bacterium with extreme
radio-resistant capability. It exhibits radiation survival up to 15,000 Gy while still be
able to grow normally at 60 Gy/h. The radiation-tolerant mechanism that involves
DNA repair represents 20% of the total resistant mechanism. Meanwhile, the other
80% comes from antioxidants. Since D. radiodurans R1 was discovered in 1956, the
whole radio-resistant mechanism is not yet fully understood. Mycosporine-like amino
acids (MAAs) are a group of 40 or more compounds that have antioxidant, growth
stimulation and UV protective properties found in many microorganisms. In D.
radiodurans R1, 3-dehydroquinate synthase (DHQ) gene annotated in chromosome 1
encodes the precursor for all MAAs. In this study, a significant amount of MAAs was
found in D. radiodurans R1 after treatment with a different type of UV radiations,
namely; UVA (360 nm) 6W and 100 W, and UVC (254 nm) 6W at a period of 12 to
48 hours. The RNA and MAAs were isolated from the UV-treated D. radiodurans R1.
RT-qPCR experiment of the DHQ gene resulted in a significant increase in the number
of expression fold from 4 to 9273 fold. Consequently, specific MAAs were
identified using time-of-flight mass spectrometry (TOF-MS). They are mycosporinetaurine,
mycosporine-glutamine, mycosporine-glutaminol, mycosporine-glutaminolglucoside,
mycosoprine-glycine, mycosporine-2-glycine, mycosporineglycine:
glutamic acid, shinorine, mycosporine-methylamine:serine, palythine-serine,
and palythinol. The results suggested that these compounds play essential roles in D.
radiodurans R1 radio-tolerance especially Mycosporine-methylamine:serine and
palythine-serine for its expression at every UV treatment. This study may well help to
understand radiation resistance mechanism further, and it is potential to be utilized as
human protective compound against radiation risk.
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