Citation
Sarbini, Shahrul Razid and Mohd Zaman, Siti Aisyah and Rawi, Muhamad Hanif and N.H., Arshad
(2018)
Resistant starch evaluation and in vitro fermentation of lemantak (native sago starch), for prebiotic assessment DUPLICATE.
International Food Research Journal, 25 (3).
951 - 957.
Abstract
Resistant starch is the non-digestible portion of starch that reaches the colon and act as a prebiotic
to stimulate the activity and growth of beneficial gut microbiota. In the present study, resistant
starch content of native (lemantak), commercialized and retrograded sago and starch was
analysed, and the in vitro fermentability with known probiotics were investigated. Retrograded
starch was produced through two cycles of autoclaving and cooling steps. The resistant starch
content of each modified starch were measured based on the method approved by AOAC
2002.02. The in vitro batch fermentation was carried out with inoculation of Lactobacillus
acidophilus and Bifidobacterium animalis at 37°C for 24 hours in anaerobic condition. Total
bacteria was enumerated at 0, 6, 12 and 24 hours. Highest resistant starch content was shown
in lemantak (native sago starch) at 62.61%. Lemantak was also shown to be the most preferred
fermentation substrate with the highest number of total bacterial count at all sampling hours.
These findings suggest the potential of lemantak as a prebiotic.
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