Citation
Cheok, Choon Yoong
(2009)
Effects of Marinating Factors on the Quality of Beef Satay.
Masters thesis, Universiti Putra Malaysia.
Abstract
A series of experiments were undertaken to investigate the effects of satay marinade on
beef texture by measuring its physical changes in terms of weight gain, cooking loss and
WBSF values. Experiment I was designed to find the main substances in satay marinade
that alters the weight gain, cooking loss and WBSF values. Tenderloin beef muscle was
selected and assigned into five treatments; i.e., control, brine solution, tamarind juice,
tamarind juice plus salt and satay marinade for 30, 60, 90, 120, 150, and 180 minutes of
marinating time. Experiment II was conducted to investigate the influence of muscles type,
blade roast (BR), biceps femoris (BF) and semitendinosus (ST) on weight gain and cooking
loss, and the semitendinosus muscle was used for further assessment of tenderness. All the
beef muscles were assigned into treatments and marinating times as defined in experiment
I. Experiment III was designed to find the better marinating temperature between 4 °C (in
refrigerator) and 25 °C (room temperature) on weight gain for beef cuts in satay marinade Chuck tender beef cuts were assigned into two treatments of control and satay marinade in
two different marinating temperatures of 4 °C and 25 °C for 1st, 2nd, 3rd, 4th, 5th and 6th hour
of marinating times. The marinating time was extended to 7th, 9th, 12th and 24th hour for the
4°C in order to establish a relationship between weight gain and marinating time.
Experiment IV was conducted to find the correlation between weight gain, cooking loss
and WBSF values of tenderloin beef cuts marinated in satay marinade for 1, 2, 3, 4, 5, and
6 hours marinating time at 4°C.
Tenderloin beef cuts that were marinated in tamarind juice plus salt and satay marinade did
not show significant difference (P > 0.01) in weight gain, cooking loss and WBSF which
implied that tamarind juice plus salt were the main substances in satay marinade which
attributed to changes in measured physical parameters. Although the tamarind juice
provided the acidic condition to the marinades, it did not enhance meat water effectively
due to the inductive effect of tartaric acid. Linear regression analysis found that the
tenderness of tenderloin samples increases linearly (P < 0.05) with increasing weight gain,
but it decreases linearly (P < 0.05) with increasing cooking loss, irrespective of treatments.
The weight gain of both BF and ST were found significantly higher (P < 0.01) than BR,
but showed no significant difference (P > 0.01) between themselves in brine solution,
tamarind juice plus salt and satay marinade treatments, which implied that BF and ST
muscles from the same carcass have almost similar water binding ability. The beef chuck
cuts marinated in satay marinade at 4 °C is more flavourable in meat water enhancing compared to 25 °C (P < 0.01), but the influence of marinating temperature will only be
significance for at least two hours of marinating time. A best fitted equation was
established to express the relationship of weight gain of beef cuts marinated at 4 °C in
response to marinating time up to 24th hour. Prediction from the equation found that the
extended marinating time above six hours did not give any significant positive effect in
water enhancing for beef chuck muscles marinated in satay marinade at 4 °C, and the
weight gain rate was found at highest rate at the first two hours of marination, particularly
at the beginning of 10 minutes.
The strong negative correlation between weight gain and WBSF (P < 0.05), and positive
correlation between cooking loss and WBSF values (P < 0.01) found for tenderloin meat
marinated in satay marinade highlighted the importance of water enhancement and
cooking loss reduction in determining the tenderness of cooked tenderloin cuts. The local
tenderloin beef and chuck tender block were recommended in preparation of satay based
on data obtained. Marinating time of two hours at 4 °C is sufficient for beef cuts to
equilibrate with satay marinade in order to have maximum water enhanced
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