Citation
Cheah, Yoke Kqueen and Abdulla Seif, Maryam and Rafi, Mohamed Ikhtifar and Lim, Wei Meng and Wong, Clemente Michael Vui Ling and Tan, Annie Geok Yuan
(2018)
Effects of Open-Top Chamber on soil chemical properties and microbial growth.
Life Sciences, Medicine and Biomedicine, 2 (3).
pp. 1-6.
ISSN 2600-7207
Abstract
Global warming is the main concern in today’s century as it comes with numerous side effects to the natural environment. Open Top Chambers
(OTC) consist of metal constructions with transparent vertical side-walls and a frustum on top. An opening in the middle of the frustum allows
an air exchange to reduce temperature and humidity effects in the chamber. The size of the open top chamber which is located in Universiti
Putra Malaysia is slanted 60o
, 50cm tall, 2.08m basal diameter hexagon chamber. The Open Top Chamber experiments were carried out to
determine how much global warming has affected and is still affecting the temperature, pH, the moisture and the growth of the microbes in
the tropical soil. The aim of this study is to elucidate the effects of temperature increase on the soil microbes’ population and on the pH of the
soil. The study was conducted to observe the effect of heat on the population of soil microbes and the pH of the soil which was collected on
the same day for 6 consecutive months. The microbes from the samples were grown on agar plates. The population of microbes on the plates
were used as values were for Colony Forming Unit (CFU) value calculations. The effects of OTCs on mean temperature showed a large range
of CFU values throughout the 6 months but did not differ significantly between studies. Increases in mean monthly and diurnal temperature
were strongly related, indicating that the presence of warming effect by the OTCs. Such predictive power allows a better mechanistic
understanding of observed biotic response to experimental warming. This study will be useful for the understanding of the global warming
effect on microbes. The Open Top Chamber experiment has proven to be one of the effective model for global warming research and data
collected especially on the growth of soil microbial obtained would be of great use for further experiments.
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