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Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K


Citation

Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource Technology, 96 (4). pp. 429-436. ISSN 0960-8524; ESSN: 1873-2976

Abstract / Synopsis

The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
DOI Number: https://doi.org/10.1016/j.biortech.2004.06.012
Publisher: Elsevier
Keywords: Organic solvent-tolerant protease; Physical factors; Pseudomonas aeruginosa
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 07 Jun 2010 06:53
Last Modified: 28 Sep 2016 07:03
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2004.06.012
URI: http://psasir.upm.edu.my/id/eprint/7075
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