Citation
Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar
(2005)
Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K.
Bioresource Technology, 96 (4).
pp. 429-436.
ISSN 0960-8524; ESSN: 1873-2976
Abstract
The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media.
Download File
Official URL or Download Paper: http://www.sciencedirect.com/science/article/pii/S...
|
Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Science and Environmental Studies |
| DOI Number: | https://doi.org/10.1016/j.biortech.2004.06.012 |
| Publisher: | Elsevier |
| Keywords: | Organic solvent-tolerant protease; Physical factors; Pseudomonas aeruginosa |
| Depositing User: | Erni Suraya Abdul Aziz |
| Date Deposited: | 07 Jun 2010 06:53 |
| Last Modified: | 28 Sep 2016 07:03 |
| Altmetrics: | http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2004.06.012 |
| URI: | http://psasir.upm.edu.my/id/eprint/7075 |
| Statistic Details: | View Download Statistic |
Actions (login required)
 |
View Item |