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High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica


Citation

Mat Radzi, Salina and Basri, Mahiran and Salleh, Abu Bakar and Ariff, Arbakariya and Mohammad, Rosfarizan and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha (2005) High performance enzymatic synthesis of oleyl oleate using immobilised lipase from Candida antartica. Electronic Journal of Biotechnology, 8 (3). pp. 291-298. ISSN 0717-3458

Abstract

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P ≥ 3.5, temperature; 40-50ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90% yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95% liquid wax esters were produced.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
DOI Number: https://doi.org/10.2225/vol8-issue3-fulltext-4
Publisher: Pontificia Universidad Católica de Valparaíso
Keywords: Oleyl oleate; Immobilised lipase; Esterification; Liquid wax ester
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 04 Jun 2010 06:43
Last Modified: 28 Sep 2016 02:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.2225/vol8-issue3-fulltext-4
URI: http://psasir.upm.edu.my/id/eprint/7056
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