UPM Institutional Repository

Screening of Aloe vera medium with different carbon and nitrogen sources for Lactobacillus acidophilus cultivation using fractional factorial design (FFD)


Citation

Mohammed Yaziz, Hifa Nazirah and Salehuddin, Nur Syahirah and Tengku Zainal Mulok, Tengku Elida and Abd Hamid, Umi Marshida and M. N., Rohana and Ahmad, Siti Aqlima and Dahalan, Farrah Aini and Abdul Khalil, Khalilah (2013) Screening of Aloe vera medium with different carbon and nitrogen sources for Lactobacillus acidophilus cultivation using fractional factorial design (FFD). In: 2013 IEEE 3rd International Conference on System Engineering and Technology (ICSET 2013), 19-20 Aug. 2013, Faculty of Electrical Engineering, Universiti Teknologi Mara, Shah Alam. (pp. 48-52).

Abstract

The aim of this research was to optimize the cultivation medium for economic production of a probiotic bacterium, Lactobacillus acidophilus using Aloe vera medium with different carbon (glucose, fructose and sucrose) and nitrogen (yeast extract, meat peptone, ammonium sulphate and urea) sources. Screening step was performed using 28 1/16 fractional factorial design (FFD) to investigate the significant effect of 8 factors used in this study on the biomass production expressed in log10 cfu/ mL. Biomass production was measured based on total plate count method for enumeration of viable cells. In the process of screening, the concentration range of Aloe vera, carbon and nitrogen used were from 1-2% (w/v), 1-2% (w/v) and 0.5-1% (w/v), respectively. The maximum biomass production was obtained with 11.816 log10 cfu/mL. It was shown that glucose, Aloe vera gel, combination of glucose and fructose and combination of glucose and ammonium sulphate were resulted significant (p <; 0.05) effect towards to the response, biomass production.


Download File

[img]
Preview
Text (Abstract)
Screening of Aloe vera medium with different carbon and nitrogen sources for Lactobacillus acidophilus cultivation using fractional factorial design (FFD).pdf

Download (37kB) | Preview

Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1109/ICSEngT.2013.6650141
Publisher: IEEE
Keywords: Optimization; Aloe vera gel; Lactobacillus acidophilus; FFD
Depositing User: Nabilah Mustapa
Date Deposited: 15 Apr 2020 16:56
Last Modified: 15 Apr 2020 16:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1109/ICSEngT.2013.6650141
URI: http://psasir.upm.edu.my/id/eprint/69510
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item