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Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction


Citation

Tan, Sheau Wei and Omar, Abdul Rahman and Ideris, Aini and Yusoff, Khatijah and Tan, Wen Siang (2004) Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction. Acta Virologica, 48. pp. 23-28. ISSN 0001-723X; ESSN: 1336-2305

Abstract

A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes the melting temperature (Tm) ranged from 86°C to 87°C. The sensitivity of the real time PCR was compared with the reverse transcription (RT) – nested PCR enzyme – linked immunosorbent assay (ELISA, RT-nested PCR ELISA). Whereas the detection limit of the real time PCR was 10 pg DNA, the RT-nested PCR ELISA and conventional PCR could only detect up to 1 ng and DNA, respectively. Thus the real time PCR offers a sensitive, rapid and convenient method for screening large number of NDV specimens.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science and Environmental Studies
Faculty of Veterinary Medicine
Publisher: AEPress
Keywords: ELISA; Newcastle disease virus; SYBR Green I; Real time PCR; RT-nested PCR ELISA
Depositing User: Users 17 not found.
Date Deposited: 24 Nov 2008 15:50
Last Modified: 20 Aug 2018 04:38
URI: http://psasir.upm.edu.my/id/eprint/692
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