Citation
Abstract
A two-step SYBR Green I real time polymerase chain reaction (PCR, real time PCR) for the detection of Newcastle disease virus (NDV) was developed. A melting curve analysis was performed to distinguish specific from non-specific products and primer dimmers. Regardless of different virus pathotypes the melting temperature (Tm) ranged from 86°C to 87°C. The sensitivity of the real time PCR was compared with the reverse transcription (RT) – nested PCR enzyme – linked immunosorbent assay (ELISA, RT-nested PCR ELISA). Whereas the detection limit of the real time PCR was 10 pg DNA, the RT-nested PCR ELISA and conventional PCR could only detect up to 1 ng and DNA, respectively. Thus the real time PCR offers a sensitive, rapid and convenient method for screening large number of NDV specimens.
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Additional Metadata
| Item Type: | Article |
|---|---|
| Divisions: | Faculty of Science and Environmental Studies Faculty of Veterinary Medicine |
| Publisher: | AEPress |
| Keywords: | ELISA; Newcastle disease virus; SYBR Green I; Real time PCR; RT-nested PCR ELISA |
| Depositing User: | Users 17 not found. |
| Date Deposited: | 24 Nov 2008 15:50 |
| Last Modified: | 20 Aug 2018 04:38 |
| URI: | http://psasir.upm.edu.my/id/eprint/692 |
| Statistic Details: | View Download Statistic |
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