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Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers


Citation

Khaledi, Kourosh Jome and Panandam, Jothi Malar and Abdul Aziz, Maheran and Siraj, Siti Shapor (2009) Reliable and fast molecular sexing in Bos indicus cattle using amelogenin gene primers. Journal of Applied Animal Research, 35 (1). pp. 83-85. ISSN 0971-2119

Abstract

To investigate reliability of amelogenin gene primer pair for exon 5, SE47/SE48 for sex differentiation in Bos indicus three male and three female Brakmas (B. indicus) cattle were tested. The SE 47/48 primer pair generated PCR products of fragment length 280 bp for the females (AMELX) and fragments of 280 bp (AMELX) and 213 bp (AMELY) for the males. Thus the SE47/48 primer pair is not only reliable for sex differentiation in B. indicus but may also be used to differentiate the B. indicus and B. taurus males.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Keywords: Amelogenin, Bos indicus, Sex differentiation
Depositing User: Norhazura Hamzah
Date Deposited: 19 May 2010 08:14
Last Modified: 28 Sep 2015 02:48
URI: http://psasir.upm.edu.my/id/eprint/6568
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