Citation
Hosseini, Seyed Davood and Omar, Abdul Rahman and Ideris, Aini and Ali, Abdul Manaf
(2004)
Expression of Iran isolate IBDV VP2 and VPX protein in prokaryotic expression system.
In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 366-367).
Abstract
A serotype I very virulent infectious bursal disease virus (vvIBDV) isolated in Iran, SDH1 IBDV isolate, was subjected to express in prokaryotic system. The VP2 and VPX genes of the isolate were amplified by reverse transcription-polymerase chain reaction, cloned, sequenced and expressed in Escherichia coli system as a fusion protein with six-histidine tag. Protein bands with expected molecular weight of 48KD and 51KD were detected by directed protein staining and western blotting. These expressed protein can be considered as a candidate for serological tests instead of whole virion, and also provide a starting point in the design of alternatives to live IBDV vaccine for prevention of infectious bursal disease.
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Additional Metadata
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | Faculty of Food Science and Biotechnology Faculty of Veterinary Medicine |
| Publisher: | Universiti Putra Malaysia Press |
| Keywords: | IBDV; Prokaryotic system; Expression; Iran |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 04 Sep 2018 04:04 |
| Last Modified: | 04 Sep 2018 04:04 |
| URI: | http://psasir.upm.edu.my/id/eprint/65118 |
| Statistic Details: | View Download Statistic |
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