Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus

Citation

Hamzah, Hairul Aini and Omar, Abdul Rahman and Bejo, Mohd Hair and Ideris, Aini (2004) Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus. In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 226-227).

Abstract

The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR was at least 10 fold more sensitive than the conventional gel electrophoresis. Thus, real-time PCR using SYBR Green 1 offers a sensitive, rapid and convenient method for screening large number of IBDV.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Veterinary Medicine
Publisher:	Universiti Putra Malaysia Press
Keywords:	Chicken; IBDV; SYBR Green I; Real-time RT-PCR
Depositing User:	Nabilah Mustapa
Date Deposited:	04 Sep 2018 04:01
Last Modified:	04 Sep 2018 04:01
URI:	http://psasir.upm.edu.my/id/eprint/65106
Statistic Details:	View Download Statistic

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