Infectivity of CD4+ T cells and CD8+ T cells against IBDV in chicken

Infectious bursal disease virus (IBDV) is a viral immunosuppressive disease found in chickens chickens, targeting mainly the Bursa of Fabricious, a major lymphoid organ in flocks. The disease causes egregious economic loss to commercial poultry industry since its emergence. Therefore, understanding on the fundamental of immune cell interaction with the virus is crucial and may greatly aid in vaccine development. To date, the effect and interaction of IBDV with chicken immune cell is still uncertain. This study aims to investigate the infectivity of IBDV against chicken CD4+ T-cells and CD8+ T-cells. The CD4+ T-cells and CD8+ T-cells population were isolated from spleen using MACS magnetic cell isolation method. The purity of isolated cells were tested using flowcytometry. Subsequently, the primary splenic CD4+ T-cells and CD8+ T-cells were infected with very virulent (vvIBDV) strain, UPM0081 and vaccine strain, D78 vaccine strain. Samples were then harvested at different time point for viral load study using real time PCR method by detecting the expression level of the IBDV viral protein 4 (VP4) antigen gene. This research may leads to the understanding of the role of specific T cells its along IBDV virus infection in chicken. This study suggest that CD4+ T-cells and CD8+ T-cells may not be susceptible to IBDV infection as viral load study consistently shown non-detectable results for the presence of VP4 gene. The research may as well provide a clue to better treatment and the virus effect on chicken which can reduce lives loss due to IBDV infection in flock.

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