Citation
Abstract
Fowlpox is a viral disease of chicken caused by fowlpox virus (FWPV). Compared to other viral infectious diseases, studies on the interaction of antigen-presenting cells (APC) such as dendritic cells (DCs) and macrophage with FWPV remains limited. Therefore, this work was aimed to characterize cytokine responses upon infection of recombinant fowlpox viruses (rFWPV) expressing H5 gene of avian influenza virus (AIV) compared to wild type FWPV on chicken DCs and macrophage cells. Chicken bone marrow cells were first isolated. The cells were differentiated into DCs (GM-CSF and IL-4 acted as supplements in RPMI media), or into macrophages (no supplements added). In order to quantify chicken IL-15 and IL-18 cytokine expression, qPCR assays based on real-time analysis were performed. Data suggested that rFWPV induced higher expression for IL-15 and IL-18 on macrophage while wild type FWPV induced higher expression for IL-15 and IL-18 on DC.
Download File
|
Additional Metadata
Item Type: | Conference or Workshop Item (Paper) |
---|---|
Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Veterinary Medicine Institute of Bioscience |
Publisher: | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
Keywords: | Dendritic cells; Macrophage cells; Fowlpox virus |
Depositing User: | Nabilah Mustapa |
Date Deposited: | 03 Sep 2018 04:49 |
Last Modified: | 03 Sep 2018 04:49 |
URI: | http://psasir.upm.edu.my/id/eprint/65042 |
Statistic Details: | View Download Statistic |
Actions (login required)
![]() |
View Item |