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Bacteriophage as carrier for multiple DNA vaccine in poultry


Citation

Al-Tayyar, O. B. and Siang, T. W. and Omar, Abdul Rahman and Ideris, Aini (2015) Bacteriophage as carrier for multiple DNA vaccine in poultry. In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 129-131).

Abstract / Synopsis

Bacteriophage-mediated immunization is a novel vaccine technology for DNA-vaccine delivery which has many advantages over traditional vaccines. The risk of reversion is absent, it can induce both humoral and cellular immunity, large-scale production is cheaper and its transportation is easier as there are no particular storage requirements. After viral RNA extraction, the specific genes were amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The amplified PCR products were inserted into bacteriophage lambda vector and successful recombinant were confirmed by white-blue screening, colony-PCR and sequencing. The aim of this study is to produce a multiple bacteriophage-mediated DNA-vaccine for poultry production.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Institute of Bioscience
Publisher: Faculty of Veterinary Medicine, Universiti Putra Malaysia
Keywords: Bacteriophage-mediated; Multiple bacteriophage-mediated DNA
Depositing User: Nabilah Mustapa
Date Deposited: 03 Sep 2018 12:41
Last Modified: 03 Sep 2018 12:41
URI: http://psasir.upm.edu.my/id/eprint/65019
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