Citation
Al-Tayyar, O. B. and Siang, T. W. and Omar, Abdul Rahman and Ideris, Aini
(2015)
Bacteriophage as carrier for multiple DNA vaccine in poultry.
In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 129-131).
Abstract
Bacteriophage-mediated immunization is a novel vaccine technology for DNA-vaccine delivery which has many advantages over traditional vaccines. The risk of reversion is absent, it can induce both humoral and cellular immunity, large-scale production is cheaper and its transportation is easier as there are no particular storage requirements. After viral RNA extraction, the specific genes were amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The amplified PCR products were inserted into bacteriophage lambda vector and successful recombinant were confirmed by white-blue screening, colony-PCR and sequencing. The aim of this study is to produce a multiple bacteriophage-mediated DNA-vaccine for poultry production.
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Additional Metadata
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | Faculty of Biotechnology and Biomolecular Sciences Faculty of Veterinary Medicine Institute of Bioscience |
| Publisher: | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| Keywords: | Bacteriophage-mediated; Multiple bacteriophage-mediated DNA |
| Depositing User: | Nabilah Mustapa |
| Date Deposited: | 03 Sep 2018 04:41 |
| Last Modified: | 03 Sep 2018 04:41 |
| URI: | http://psasir.upm.edu.my/id/eprint/65019 |
| Statistic Details: | View Download Statistic |
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