Citation
Khanh, Nguyen Phuc and Tan, Sheau Wei and Yeap, Swee Keong and Satharasinghe, Dilan Amila and Bejo, Mohd Hair and Omar, Abdul Rahman
(2015)
Characterization of infectious bronchitis viruses isolated from commercial poultry farms in Malaysia.
In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 52-54).
Abstract
Infectious bronchitis virus (IBV) primarily causes a highly infectious bronchitis (IB) disease in commercial chickens. Virus isolation and identification is necessary for diagnosis of the disease. A total of 6 IBV isolates from commercial poultry farms in Malaysia were isolated by inoculation in 9 day-old embryonated SPF eggs. After few passages, the embryos showed typical lesions of IB such as stunted and curled cross with feather dystrophy. The isolated IBV were characterized by RT-PCR followed by DNA sequencing of partial S1 gene. Sequencing and phylogenetic analysis showed that all the IBV isolates can be divided into 2 distinct groups. In group I (IBS037A, IBS037B, IBS051 and IBS052) shared 70% similarity with Group II (IBS130 and IBS131). However, group II viruses were genetically closed to QX-like IBV isolates with similarity of 93%. Meanwhile, Group I that formed a distinct branch of the phylogenetic tree has genetic distance ranged from 30% to 36% with 793/B, Mass-type, Taiwan-type and QX-like viruses.
Download File
Additional Metadata
Actions (login required)
 |
View Item |
![[img]](http://psasir.upm.edu.my/style/images/fileicons/text.png)