Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry

Citation

Haddadi, Fatemeh and Abdul Aziz, Maheran and Saleh, Ghizan and Abdul Rashid, Azmi and Kamaladini, Hossein (2008) Effect of TDZ and BAP on shoot proliferation from in vitro shoot tips of strawberry. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 443-446).

Abstract

An experiment was conducted to induce shoot proliferation from shoot tips derived from runner tips of strawberry cv. Camarosa. The shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with TDZ and BAP, either alone or in combination. The concentrations used were 0 to 8 μM TDZ and 0 to 27 μM BAP. MS medium supplemented with a moderate concentration of 4 μM BAP in combination with 2 μM TDZ was optimum for strawberry shoot proliferation from shoot tips derived from runner tips. In this combination of cytokinins, a 100 % shoot proliferation with a mean number of 14.6 shoots per explant was produced. This regeneration protocol is suitable for mass propagation of planting materials for commercial cultivation of strawberry and for the recovery of transgenic strawberry plants following genetic transformation.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Agriculture
Publisher:	Sarawak Biodiversity Centre
Keywords:	TDZ; BAP; MS medium; Strawberry shoots proliferation
Depositing User:	Nabilah Mustapa
Date Deposited:	04 Jul 2018 02:31
Last Modified:	04 Jul 2018 02:31
URI:	http://psasir.upm.edu.my/id/eprint/64278
Statistic Details:	View Download Statistic

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