UPM Institutional Repository

Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus


Citation

Muhammad, Umar Abubakar and Tham, Lik Gin and Perumal, Natarajan and Mohd Daud, Hassan and Yasid, Nur Adeela and Abd. Shukor, Mohd. Yunus (2016) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus. Bioremediation Science and Technology Research, 4 (2). pp. 6-10. ISSN 2289-5892

Abstract

Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078, 0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature.


Download File

[img]
Preview
Text (Abstract)
Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus.pdf

Download (35kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
Publisher: Hibiscus Publisher
Keywords: Clarias batrachus; Acetylcholinesterase; Heavy metals; Inhibitive assay
Depositing User: Nabilah Mustapa
Date Deposited: 08 Jun 2018 00:51
Last Modified: 08 Jun 2018 00:51
URI: http://psasir.upm.edu.my/id/eprint/64020
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item