Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

Citation

Muduli, Saradaprasan and Chen, Li-Hua and Li, Meng-Pei and Heish, Zhao-wen and Liu, Cheng-Hui and Kumar, Suresh and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Benelli, Giovanni and Murugan, Kadarkarai and Wang, Han-Chow and Chen, Da-Chung and Hsu, Shih-Tien and Chang, Shih-Chang and Higuchi, Akon (2017) Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides. Journal of Polymer Engineering, 37 (7). 647 - 660. ISSN 0334-6447; ESSN: 2191-0340

Abstract

The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

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Additional Metadata

Item Type:	Article
Divisions:	Faculty of Medicine and Health Science
DOI Number:	https://doi.org/10.1515/polyeng-2016-0193
Publisher:	Walter de Gruyter GmbH
Keywords:	Biomaterial; Cell culture; Elasticity; Poly(vinyl alcohol); Stem cell
Depositing User:	Mohd Hafiz Che Mahasan
Date Deposited:	26 Sep 2018 01:51
Last Modified:	26 Sep 2018 01:51
Altmetrics:	http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1515/polyeng-2016-0193
URI:	http://psasir.upm.edu.my/id/eprint/63287
Statistic Details:	View Download Statistic

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